10 Resources

专题介绍：单细胞RNA-seq被评为2018年重大科研进展，但实际上这是老技术。2015年，商品化单细胞RNA测序流程已经建立，成果发表在Cell上。今年井喷式发文章，关注点那么高，是因为最近这项技术全面商品化了。

10.1 Orchestrating Single-Cell Analysis with Bioconductor

OSCA

10.2 scRNA-seq Protocols

• 10x Chromium
• Single Cell Genomics Library Structure
• SMART-seq2
• CELL-seq
• Drop-seq
• UMI
• STRT-Seq

10.3 External RNA Control Consortium (ERCC)

ERCCs

10.4 scRNA-seq analysis tools

Extensive list of software packages (and the people developing these methods) for single-cell data analysis:

• awesome-single-cell

Tallulah Andrews’ single cell processing scripts:

• scRNASeqPipeline

Various tools from 10x Genomics:

• Cell Ranger
• Space Ranger

10.5 scRNA-seq public datasets

• Hemberg group’s public datasets
• scRNAseq R package – 64 public datasets